The Persian Speech Database Farsdat comprises the recordings of 300 Iranian speakers, who differ from each other with regards to age, sex, education level, and dialect (10 dialect regions of Iran were represented: Tehrani, Torki, Esfahani, Jonubi, Shomali, Khorassani, Baluchi, Kordi, Lori, and Yazdi). Each speaker uttered 20 sentences in two sessions, and 100 of these speakers uttered 110 isolated words. 6000 utterances were segmented and labelled phonetically and phonemically manually, including 386 phonetically balanced sentences, using IPA characters. The acoustic signal has been stored with a Wave file standard, so that it can be used by any other application software. The used sampling frequency reaches 22.5 KHz, and the signal-to-noise ratio 34 dB. The ambiguities in segmentation have been solved by reference to the corresponding spectrograms extracted from DSP sona-Graph KAY 5500.